Qiagen clinical insight bioinformatics software and. For disruption of up to 48 or 192 biological samples 48. Tissuelyser adapter set 2 x 96 from qiagen selectscience. Cuttingedge technology, unique features and algorithms widely used by scientific leaders in industry and academia make it easy to overcome challenges associated with data analysis. These are available online in convenient and compact pdf. Qiagen clc genomics workbench qiagen digital insights. Qiagen clc genomics workbench is a powerful solution that works for everyone, no matter the workflow. The ngs module supports the analysis of qiagen s qiaseq ngs library kits and target enrichment panels. Qiagen tissuelyser ii at 30 fs hz three times for 30 seconds, with placement of the samples on ice in between bead beating steps. Checkout popular qiagen categories laboratory equipment manuals. Comparison of dna extraction methods for human gut. Tissueruptor tissuelyser lt tissuelyser ii throughput samplesrun 1 up to 12 up to 48 or 192 disruption technology rotorstator bead mill bead mill disruption time 30 seconds 40 seconds to 5 minutes 20 seconds to 5 minutes table 1.
Standard operating procedure sop for dnarna extraction. For mouse samples, one fecal pellet was placed into a 2 ml sterile roundbottom tube containing 500. For disruption of up to 48 or 192 biological samples 48 pages. Laboratory equipment qiagen tissuelyser ii user manual 48 pages. I asked precellys similar question and they suggested 2 3 cycles 40 second each with ck28 beads ceramic 2. Excise the tissue sample from the animal or remove it from storage. For each method, an extraction blank pcrgrade water was used to ascertain potential kit andor reagent contamination. Generates a prerun report to assist with workspace setup for the current run.
Qiagen provides stainless steel beads and tungsten carbide beads for use in sample disruption with tissuelyser systems. The tissuelyser adapter set 2 x 24 contains a pair of tl reactiontube holders, 24tube. Ive read many protocols, help guides and specifically the troubleshooting guide in the kit protocol, but the number one piece of advice im seeing is to use the tissuelyser andor tissueruptor. Processing of up to 2 x 96 samples takes as little as 2 5 minutes. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Should any product fail to perform satisfactorily due to any reason other. See the tissuelyser lt handbook or the tissuelyser handbook. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation, such as the qiasymphony sp. The powerfood microbial dna isolation kit was then used as per the manufacturers instructions. May 2016 tissuelyser ii user manual gtac home page.
Does anyone use the tissuelyser ii qiagen equipment to. Do not continue to use the tissuelyser ii or tissuelyser. Preparation of cytosolic and microsomal fractions was performed as described previously. Advanced analysis, workflow and interpretation software accessing genomic and clinical knowledge from over 20 million references. Carbonyl reduction of nnk by recombinant human lung enzymes. If the tissuelyser ii is not in use, switch it off at the power switch. Various rat tissues 25 mg each stabilized in allprotect tissue reagent were disrupted using the tissuelyser lt or tissuelyser ii. Comparative evaluation of dna extraction methods from feces.
The software evaluates genomic variants in the context of published biomedical literature, professional association guidelines, publicly available databases and annotations, drug. I am sure you can buy equivalent beads at vwr or fisher. Laboratory equipment qiagen tissuelyser ii user manual. The qiagen tissuelyser lt sample disruptor provides quick and efficient disruption in up to 12 samples. Qiagility laboratory equipment pdf manual download.
Tissuelyser 3 mm bead dispenser, 96well from qiagen. Tissuelyser handbook university of texas at arlington. Sample disruption tissuelyser ii for medium to highthroughput sample disruption for molecular analysis 85300 tissuelyser lt for low to mediumthroughput sample disruption for molecular analysis 85600 powerlyzer 24 homogenizer 110220 v for the most efficient and complete lysis and. Simultaneous disruption and homogenization is achieved through highspeed shaking of samples in 2 ml.
We stiil use pestle and mortar which is very timeconsuming and we would like to speed. This german made shaker appears in good condition without cracks in the plastic cover or other damage to the housing or workings. Determine the amount of tissue and place it into the qiazol lysis reagent. Various rat tissues were disrupted using the tissuelyser lt or. Samples were processed on a tissuelyser ii qiagen, venlo, netherlands for 10 min. Composition and method for disrupting tissue material download pdf. Qiagen focuses on breaching great success and development in their company to better serve their consumers. Evaluating the impact of dna extraction method on the. Extraction buffer exb plus provided in the qproteome ffpe kit qiagen cat. Mechanical cell rupture was performed using a qiagen tissuelyser ii at 30 hz for 2 x 50 s, as applicable. Tissuelyser adapter set 2 x 24 from qiagen selectscience.
Lung 60 50 40 30 20 10 0 tissuelyser lt tissuelyser ii liver heart brain skin 400 300 200 100 0 heart brainlung tissuelyser lt tissuelyser ii figure 6. Tissuelyser ii the tissuelyser ii provides highthroughput processing for simultaneous, rapid, and effective disruption of up to 192 biological samples, including all types of animal tissue. Qiagen qiaamp kits provide a fast, easy, and reliable method to purify total dna from small quantities of human tissue or blood. Disruption and homogenization using the tissuelyser lt or tissuelyser ii. Does anyone have experience with tissue lyser from qiagen. Manuals bioinformatics software and services qiagen. Used qiagen mm300 tissuelyser shaker mixer grinder agitator. Tissuelyser ii tissuelyser ii delivers medium to highthroughput disruption of up to 2 x 96 samples of tissue, plant material, yeast or bacteria in less than 5 minutes.
Purification of total rna, including small rnas, from human leukocytes 38 appendix e. The tissuelyser enables fast and uniform disruption of animal and human tissues, plant tissues, bacteria, and yeast in various sample volumes in several formats. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. The lysate generated from this step was then prepared using the high pure pcr template preparation kit. Use of these in next generation sequencing ngs is a priority. Qiagen offers adapter sets for 2 x 96 collection microtubes 1.
Safety data sheets sds for any qiagen or preanalytix product can be downloaded from. Qiacube ht laboratory equipment pdf manual download. The tissuelyser lt is compact so you can keep it nearby for easy access. This throughput matches that of the qiacube, which automates sample preparation using trusted qiagen spincolumn kits. These are available online in convenient and compact pdf format at.
This small bead mill has high throughput with highspeed shaking for the disruption or homogenization of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. Highspeed shaking of sample material and a single bead in a collection microtube or 2 ml microcentrifuge tube for a few minutes allows rapid and efficient disruption of the sample. For efficient disruption of up to 48 or 192 samples in parallel on the tissuelyser ii. Qiagen tissuelyser ii sample disruption 11843 youtube. Sample to insight beadbasedhomogenization for unbiased nucleic acid representation metal 2. Apr 28, 2017 tissuelyser lt tissuelyser lt is a small bead mill that enables low to mediumthroughput sample disruption, of up to 12 samples at the same time.
Sequencebased analysis of the bacterial and fungal. Qiagen clc genome finishing module is an addon to qiagen clc genomics workbench, designed to accelerate and simplify genome finishing, and make this process accessible to life scientist without deep understanding of bioinformatics. Qiaex ii handbook qiaex ii agarose gel extraction protocol. It ramps up to 30 shakes per second and the timer works as it should. The kit uses qiagens secondgeneration inhibitor removal technology irt, and is intended for use with. The realtime pcr modules transform threshold cycle c t values to calculated results for gene and mirna expression, somatic mutation detection and copy number measurements. It has been stored under unfavorable conditions for a prolonged period. Equipnet is constantly receiving used qiagen equipment through. Keep the insert of the tissuelyser lt adapter at room temperature. The remaining steps were carried out according to the manufacturers recommendations, and the dna eluted in 100 ul elution buffer. Real time access and analysis of over 40 genomic and clinical databases covering over 33,000 diseases. Qiagen is the leading provider of innovative sample and assay technologies, enabling the isolation and.
Cranberry microsatellite marker development from assembled. Subsequently, the samples were heated at 95c for 15 minutes. Jun 02, 2014 add the appropriate volume of buffer rlt see table 8, and homogenize by passing the lysate at least 5 times through a blunt 20gauge needle fitted to an rnasefree syringe. There are minor cosmetic scratches on the top plate, but nothing serious. The purchaser must determine the suitability of the product for its particular use. Place the tubes into the insert of the tissuelyser lt adapter, and incubate at room temperature for 2 min to avoid freezing of lysis buffer in step 3.
Tissuelyser ii laboratory equipment pdf manual download. Ursodeoxycholic acid enriches intestinal bile salt hydrolase. We used 2030mg of tissue material in 600ll of buffer rlt plus with 6ll bmercaptoethanol. Aug 24, 2018 a detailed investigation of the porcine skin and nose microbiome using universal and staphylococcus. For highly specific and sensitive multiplex pcr without optimization requirements. For disruption of up to 48 or 192 biological samples. The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic. It has been subjected to severe transport stresses. All instructions and protocols in this handbook apply to both the tissuelyser ii and the. Bioinformatics software and services qiagen digital insights.
In addition, they provide a fast and simple method for the preparation of dna and column purified rna from human tissues. Qiagen retsch tissuelyser ii bead mill for sale 591 used retsch grindomix gm 200 knife mill sale advatec low friction thermal barrier coatings based on. Its wide variety of features are presented in an intuitive graphical userinterface, for which advanced computer skills are not required. Following incubation at 65 c and chloroform extraction, the crude dna extract was digested for 30 min at 37 c with rnase a 20 lg prior to isopropanol precipitation.
Equipnet is constantly receiving used qiagen equipment through our exclusive contracts with our clients. Qiagen allprep kits are designed to isolate dna and total rna from small quantities of starting material. Add 1 gel volume isopropanol to the sample and mix. Disruption and homogenization using the tissuelyser lt. Disruption and homogenization using the tissuelyser ii gives yields comparable. The tissuelyser lt is a small bead mill which provides fast, effective disruption of up to 12 samples at the same time.
Transfer up to 30mg fresh or frozen tissue to the precooled tubes and incubate for another 15 min on dry ice. This easytouse instrument automates purification of dna, rna, and protein from 196 samples. Tissue homogenization was performed with a qiagen tissuelyser ii in 2 ml tubes with a 5mm bead at 22 hz for 2 min. Qiagen clinical insight qci is an evidencebased decision support software intended as an aid in the interpretation of variants observed in genomic sequencing data.
The samples were subjected twice to bead beating using the qiagen tissuelyser ii at a frequency of 30 hz for 1 min each with a 5 min rest on ice in between, and then centrifuged. For highthroughput disruption of biological samples pdf 89kb. Qiagen multiplex pcr kit, x 50l rxns, genomic dna and cdna sample, pcr amplification reaction, 5 3 exonuclease enzyme activity, easytouse and costeffective, for highly specific and sensitive multiplex pcr without optimization requirements, includes 2x qiagen. The system provides the same highquality results as other trusted qiagen purification technologies. The geneglobe data analysis center is a complimentary resource for analyzing realtime pcr or ngs data. The initial step in the performance of the assay was the performance of realtime pcr, using the lightcycler 480 roche diagnostics. Rna extraction from plants using the rneasy plant mini kit. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation in cell culture. The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Do not continue to use the tissuelyser ii or tissuelyser accessory if it is damaged. Alternatively, a grinding jar set can be used to process large samples. Qiagen clc main workbench bioinformatics software and.
Tissuelyser ii user manual 052016 7 it or the line power cord appears to be damaged. All microsomes were washed once with trisbuffered saline ph 7. If using the tissuelyser lt or tissuelyser ii, add the stainless steel beads of choice 1 x 5 mm or 2 x 7mm to the tube before the addition of qiazol. Aug 28, 2018 genomic dna gdna extracted from fresh, freezedried, and silica geldried plant tissue. Extraction was optimised with a 10 min 70 c incubation quigley et al. Archival tissues represent a rich resource for clinical genomic studies, particularly when coupled with comprehensive medical records.
Supplemental material and methods specimen collection and. View and download qiagen qiagility user manual online. Storage, quantification, and determination of quality of rna 41 ordering information 44. Rnalater rna stabilization reagent qiazol lysis reagent. Quality assessment included total yield, percent dsdna. Qiagen offers advanced and innovative solutions for all of your assay and sample equipment needs. Add the appropriate volume of buffer rlt see table 8, and homogenize by passing the lysate at least 5 times through a blunt 20gauge needle fitted to an rnasefree syringe. Nine formalinfixed paraffinembedded ffpe dna extraction methods were evaluated using twelve ffpe samples of varying tissue types. For sections from pfpe page 16 tissue, we recommend operating the tissuelyser lt or tissuelyser ii for 2 min at 25 hz. Wo20161892a1 composition and method for disrupting tissue. When using the qiagen tissuelyser lt or tissuelyser ii, process the sample according to the protocol cation of rna or multiple analytes frompurifi animal and human tissues in the tissuelyser handbook.
Lt is comparable to that achieved with the wellestablished tissuelyser ii figures 6 and 810. Tissuelyser lt adapter, 12tube from qiagen selectscience. Standard operating procedure sop for dna extraction with. Qiagen disruption systems for a range of throughputs. The purified dna is up to 50 kb in size, with fragments of 20 30 kb predominating. The purified dna and rna are ready for use in standard downstream applications such as dna amplification and expression. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for. Safety information 6 tissuelyser ii user manual 042010 with accessories approved by qiagen see section 3. Human faecal aliquots 200 mg and murine caecal content were lysed using the qiagen tissuelyser ii bead beater 25 hz for 20 minutes, with dna extracted using the.
425 1032 140 1404 25 1057 414 833 1440 1634 342 1169 622 893 1188 1223 949 264 539 709 1354 543 1047 1577 774 911 137 432 1077 845 570 818 482 600 1389